Case study: Evaluation and effect of feed with oxytetracycline industrially prepared and with a farm-based procedure on the development of Penaeus vannamei shrimp and its accumulation in muscle and hepatopancreas

Abstract

This research consisted of comparing two methods of adding oxytetracycline (OTC) in shrimp feed, as well as its development and its accumulation in muscle and hepatopancreas from Penaeus vannamei. A bioassay was carried out for 30 days using 180 juvenile shrimp testing two formulations to feed the organisms (OTC-1 and OTC-2). The formulations medicated were administered 14 days, and subsequently they were fed with a diet without antibiotic for 16 more days and water quality was determinate by physicochemical data. In shrimps the following was evaluated: Biological and physiological parameters, accumulation (C_máx) of OTC and elimination time in muscle and hepatopancreas were determined by high performance liquid chromatography (HPLC). The development of organisms showed a survival of 99.72% for OTC-1 and 100% for OTC-2. The C_máx of OTC in muscle for the OTC-1 group was 16.09 ± 1.80 µgg^-1 and the OTC-2 group was 6.62 ± 0.87 µgg^-1; in the hepatopancreas it was 105.85 ± 17.56 with (OTC-1) and 79.16 ± 15.61 µgg-1 with (OTC-2) finding an association of C_máx of OTC by the method of addition (p ≤ 0.05). The medicated shrimp feed through an industrial process (OTC-1) and the addition on the farm (OTC-2) require greater quality control to ensure the desired concentration of the antibiotic, before its administration in shrimp cultures.